Confocal microscopy enables acquisition of 2D or 3D fluorescence images as well as time-lapse images by collecting emission light only from the focus of a laser beam. Excitation light generates fluorescence throughout the specimen and out-of-focus light is eliminated by a pinhole.
Advantages: This vibratome is good for cutting agarose-embedded tissue slices from ~50 μm to ~400 μm thickness. Fixed and living tissue can be cut in the designated chambers. The vibratome uses disposable razor blades.