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Single Cell Transcriptome Analysis of Regenerating RGCs Reveals Potent Glaucoma Neural Repair Genes

EVENT: 
Weekly Seminar | Not Open to the Public
Who Should Attend: 
Researchers

Abstract

Axon regeneration holds great promise for neural repair of CNS axonopathies, including glaucoma. Pten deletion in retinal ganglion cell (RGC) promotes potent optic nerve (ON) regeneration, but only a small population of Pten-null RGCs are actually regenerating RGCs (regRGCs); most surviving RGCs (surRGCs) remain non-regenerative. Here we developed a strategy to specifically label and purify regRGCs and surRGCs respectively from the same Pten deletion mice after ON crush injury, in which they differ only in their regeneration capability. Smart-Seq2 single cell transcriptome analysis revealed multiple novel regeneration-associated genes that significantly promote axon regeneration. More strikingly, some of the axon regeneration genes dramatically protect RGC somata and axons and preserves visual function in a clinically relevant model of glaucoma, demonstrating the exciting potential of this innovative strategy to identify novel effective neural repair candidates.

Dr. Yang Hu's Figure

Publications

Li L, Fang F, Zhuang P, Feng X, Zhuang P, Huang H, Liu P, Liu L, Xu AZ, Qi LS, Cong L, Hu Y
Single Cell Transcriptome Analysis of Regenerating RGCs Reveals Potent Glaucoma Neural Repair Genes
Under revision
Fang Fang, Pei Zhuang, Xue Feng, Pingting Liu, Dong Liu, Haoliang Huang, Liang Li, Wei Chen, Liang Liu, Yang Sun, Haowen Jiang, Jiangbin Ye, Yang Hu
NMNAT2 is downregulated in glaucomatous RGCs, and RGC-specific gene therapy rescues neurodegeneration and visual function
Mol Ther. 2022 Apr 6;30(4):1421-1431. doi: 10.1016/j.ymthe.2022.01.035. Epub 2022 Jan 31.
Qizhao Wang, Pei Zhuang, Haoliang Huang, Liang Li, Liang Liu, Hannah C Webber, Roopa Dalal, Leonard Siew, Clarisse M Fligor, Kun-Che Chang, Michael Nahmou, Alexander Kreymerman, Yang Sun, Jason S Meyer, Jeffrey Louis Goldberg, Yang Hu
Mouse γ-Synuclein Promoter-Mediated Gene Expression and Editing in Mammalian Retinal Ganglion Cells
J Neurosci. 2020 May 13;40(20):3896-3914. doi: 10.1523/JNEUROSCI.0102-20.2020. Epub 2020 Apr 16.

When

Tuesday, June 14, 2022 - 12:30pm

Where

Conference Room: 
Billings Building – Rosedale Room and Zoom

More Information

Darlene White

Conditions & Recovery

Neurodegenerative Diseases icon
Worldwide, 50 million people are living with Alzheimer's and other dementias.

Research Methods: 
Axonal Regeneration