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Single Cell Transcriptome Analysis of Regenerating RGCs Reveals Potent Glaucoma Neural Repair Genes

Weekly Seminar | Not Open to the Public
Who Should Attend: 


Axon regeneration holds great promise for neural repair of CNS axonopathies, including glaucoma. Pten deletion in retinal ganglion cell (RGC) promotes potent optic nerve (ON) regeneration, but only a small population of Pten-null RGCs are actually regenerating RGCs (regRGCs); most surviving RGCs (surRGCs) remain non-regenerative. Here we developed a strategy to specifically label and purify regRGCs and surRGCs respectively from the same Pten deletion mice after ON crush injury, in which they differ only in their regeneration capability. Smart-Seq2 single cell transcriptome analysis revealed multiple novel regeneration-associated genes that significantly promote axon regeneration. More strikingly, some of the axon regeneration genes dramatically protect RGC somata and axons and preserves visual function in a clinically relevant model of glaucoma, demonstrating the exciting potential of this innovative strategy to identify novel effective neural repair candidates.

Dr. Yang Hu's Figure


Li L, Fang F, Zhuang P, Feng X, Zhuang P, Huang H, Liu P, Liu L, Xu AZ, Qi LS, Cong L, Hu Y
Single Cell Transcriptome Analysis of Regenerating RGCs Reveals Potent Glaucoma Neural Repair Genes
Under revision
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Tuesday, June 14, 2022 - 12:30pm


Conference Room: 
Billings Building – Rosedale Room and Zoom

More Information

Darlene White

Conditions & Recovery

Neurodegenerative Diseases icon
Worldwide, 50 million people are living with Alzheimer's and other dementias.

Research Methods: 
Axonal Regeneration