Western Blots

Western blots are used extensively to study post-translational modifications of the α-ketoglutarate dehydrogenase complex (KGDHC). The figure shows the separation of ketoglutarate dehydrogenase complex by blue-native gel. The complex on the gel is still enzymatically active. The result demonstrates post-translational modification of KGDHC. The blots were probed with antibodies to E1k, E2k or E3 (red) and to nitrotyrosine (green), respectively. The overlap is represented by yellow.