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Ion Mobility Mass Spectrometry Reveals Hidden Post Translational Modifications of Amyloid Beta in Alzheimer’s Disease Brain

Weekly Seminar | Not Open to the Public
Who Should Attend: 


Part of the quest to understand neurodegenerative research requires a detailed accounting and measurement of the biological components in neurological cells, tissue and biofluids. Regulation of the abundance of proteins is one mechanism of regulating function and post-translational modification (PTM) is another. PTMs are often invisible to targeted assays like ELISA or westerns this includes modifications including phosphorylation and, more recently, the hidden isomeric modifications (e.g. iso-aspartate). Here we apply quantitative proteomics and ion mobility mass spectrometry to investigate the isomerization of amyloid beta peptide from human brain tissue. To our surprise we discovered that isomerization at aspartic acid makes up over 80% of the total peptide in Alzheimer’s disease brain.  We show that the implementation of quantitative mass spectrometry and ion mobility reveal hidden biology and improve our understanding of the role of amyloid beta peptide in Alzheimer’s disease.  We will also discuss the role of protein biomarkers for the diagnosis of AD.

Dr. Blaine Roberts' Figure


Soumya Mukherjee, Keyla A Perez, Larissa C Lago, Stephan Klatt, Catriona A McLean, Ian E Birchall, Kevin J Barnham, Colin L Masters, Blaine R Roberts
Quantification of N-terminal amyloid-β isoforms reveals isomers are the most abundant form of the amyloid-β peptide in sporadic Alzheimer's disease
Brain Commun. 2021 Mar 9;3(2):fcab028. doi: 10.1093/braincomms/fcab028. eCollection 2021.
Adam P Gunn, Bruce X Wong, Catriona McLean, Chris Fowler, Peter J Barnard, James A Duce, Blaine R Roberts, AIBL Research Group
Increased glutaminyl cyclase activity in brains of Alzheimer's disease individuals
J Neurochem. 2021 Mar;156(6):979-987. doi: 10.1111/jnc.15114. Epub 2020 Jul 20.
Blaine R Roberts, Monica Lind, Aaron Z Wagen, Alan Rembach, Tony Frugier, Qiao-Xin Li, Timothy M Ryan, Catriona A McLean, James D Doecke, Christopher C Rowe, Victor L Villemagne, Colin L Masters
Biochemically-defined pools of amyloid-β in sporadic Alzheimer's disease: correlation with amyloid PET
Brain. 2017 May 1;140(5):1486-1498. doi: 10.1093/brain/awx057.


Tuesday, September 21, 2021 - 12:30pm


Conference Room: 
Online Webinar

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Darlene White

Conditions & Recovery

Neurodegenerative Diseases icon
Worldwide, 50 million people are living with Alzheimer's and other dementias.